In Thoubal, where the shutdown started in the early hours of Tuesday, women blocked the NH 102 at Mela Ground, Wangjing, Yairipok and Khangabok, police said.
Near the Mela Ground, women demonstrated in the middle of the road, while young men burnt tyres to prevent movement of vehicles.
The shutdown was strongly enforced at Khurai and Lamlong in Imphal East district.
JAC convenor L Subol said, “The agitation will continue till the youths are released.”
Three young men hailing from Thoubal district were allegedly kidnapped in Kangpokpi last week. Later, one of them was rescued, but the other two remain missing.
Accompanied by two friends, N Johnson Singh went to appear for a recruitment test at New Keithelmanbi in Manipur’s Imphal West district, but took a wrong turn, ending up in Kuki-dominated Kangpokpi district, according to police.